Document Type : Original Article
Authors
- . Latifeh Rasouli 1
- . Naheed Aryaeian 2
- . Mehran Gorjian 3
- . Mitra Nourbakhsh 3
- . Fatemehsadat Amiri 4
1 Department of Nutrition, School of Public Health, Iran University of Medical Sciences, International Campus, Tehran, Iran
2 Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran
3 Department of Biology, School of Sciences, Payame-Noor University of Mashhad, Mashhad, Iran
4 Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Abstract
BACKGROUND: Colorectal cancer is the third leading to death type of cancer in the world.
The therapeutic guideline varied between different methods. As the main therapeutic guideline
is chemotherapy, recent studies had shown utilization of natural products in combination with
conventional medication, elevate the efficiency of chemotherapeutic methods. Kombucha is a
traditional beverage obtained from the fermentation of green tea as a rich source of flavonoid
medicinal plant. This study aimed to evaluate the natural potential of combination therapy of this
natural product with doxorubicin as a chemotherapeutic agent.
MATERIALS AND METHODS: The study was performed as in vitro evaluation of biological activity
of kombucha on HCT‑116 cell line (human colon cancer cell line). The cytotoxic effect of different
kombucha beverages (fermented green tea) in comparison with green tea extract was evaluated by
dimethylthiazolyl tetrazolium bromide (MTT) assay. In the next step, anticancer activity of doxorubicin
as a general guideline chemotherapeutic agent in combination with kombucha was evaluated by
cell cycle analysis and apoptosis assay flow cytometry. Apoptotic genes expression pattern was
determined using real‑time polymerase chain reaction. The experiments were designed in three
independent replications and statistically analyzed using SPSS software.
RESULTS: The results show that kombucha compared with the green tea extract caused
more (1.2 fold) early apoptosis induction and G0/G1 phase arrest. Moreover, kombucha increased
the expression levels of p21, p53, and B‑cell leukemia/lymphoma 2 (Bcl‑2)‑associated X protein
genes (2, 2.5, and 1.5 fold, respectively) while it decreased Bcl‑2 gene expression level (5–8 fold)
compared with doxorubicin alone. Combination of kombucha with doxorubicin shows 2‑fold increased
G0/G1 phase compared with the doxorubicin treatment.
CONCLUSION: This result indicated that kombucha caused boosted anticancer activity of doxorubicin
agent. These findings suggest that kombucha may be has an assistor and useful role in colorectal
cancer treatment align with chemotherapy.
Keywords
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