Document Type : Original Article


1 Department of Hematology, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran

2 Department of Hematology, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran, Pediatric Growth and Development Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran, Department of Oncology‑Pathology, Immune and Gene Therapy Lab, Cancer Center Karolinska, Karolinska University Hospital Solna and Karolinska Institute, Stockholm, Sweden

3 Department of Biotechnology , School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran

4 Department of Pathobiology and Laboratory Sciences, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran

5 Department of Immunology, School of Medicine, Iranian Blood Transfusion Research Center, Tarbiat Modares University, Tehran, Iran


BACKGROUND: Transfusion of healthy red blood cells (RBCs) after storage is important. One of
the storage lesions on blood bags is oxidative stress. One way to prevent increased oxidative stress
is to use antioxidant nanoparticles (NPs). Superoxide dismutase (SOD) and catalase (CAT) play
an important role in antioxidant defense on RBC. poly lactic-co-glycolic acid (PLGA) is a nontoxic
biodegradable polymer that is approved by the Food and Drug Administration for drug delivery. This
study aimed to assess dose-dependent efficacy of SOD-CAT-polyethylene glycol -PLGA on RBCs
MATERIALS AND METHODS: Using a descriptive study, during 1 month, twenty donors from
Bojnourd Blood Donation Center were selected. NPs with different concentrations were injected
into the satellite bags after directing blood to them. On target days, experiments were performed on
the samples taken. Electrospray was employed to prepare SOD-CAT-PLGA NPs. Twenty packed
RBCs were isolated from the whole blood bags by the mechanical method, and certain amount of
product was transferred to the satellite bags. On days 1, 7, 14, 21, 28, and 35, bags were sampled.
Malondialdehyde (MDA), prooxidant-antioxidant balance (PAB), and Annexin V were performed on
the samples taken. The repeated measures analysis with the help of SPSS software version 20
was performed on samples.
RESULTS: MDA increased in both groups. The maximum increase in test group was seen in
concentration 12 mg (MDA Day 14, test [1.93 ± 0.3], [P MDA < 0.001]). Maximum increase in PAB
was seen in concentration 12 mg (from 444 ± 1.7 to 563 ± 2.5) (P PAB = 0.000). Furthermore,
PS expression increased in the concentration of 12 mg greater than other concentration in
consecutive (from 5.00 ± 0.8 to 22.26 ± 1.7, [P < 0.001]).
CONCLUSION: Evaluation of dose dependency showed that different concentrations of antioxidant
NPs affect RBC. This effect can be changed oxidative stress and apoptosis. Using both changes to
evaluate functional and toxicity can be helpful.


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