Journal of Education and Health Promotion 2277-9531 8 11 2018 11 01 Hepatoprotective effects of Berberis vulgaris leaf extract on carbon tetrachloride‑induced hepatotoxicity in rats 1 5 27950 EN . Maryam Tahmasebi . Heibatollah Sadeghi Medical Plants Research Center, Yasuj University of Medical Sciences, Yasuj . Habibollah Nazem Department of Biochemistry, Payam-e Noor University of Shahreza, Shahreza . Esmaiel Panahi Kokhdan Medical Plants Research Center, Yasuj University of Medical Sciences, Yasuj . Navid Omidifar Clinical Education Research Center, Zeinabieh Hospital, Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Journal Article 2023 02 15 INTRODUCTION: Hepatic sickness is a serious problem for human health. The researchers are<br />interested in using medicinal plants including barberry to cure many of these sicknesses. In this study,<br />the effect of hydroalcoholic extract of Berberis vulgaris leaf on hepatic protection was assessed in rats.<br />MATERIALS AND METHODS: Forty healthy male Wistar rats were divided randomly into five groups<br />(n = 8): Group 1 (healthy control), intraperitoneal injection of olive oil; Group 2 (hepatotoxic control),<br />intraperitoneal injection of carbon tetrachloride and daily gavage of distilled water; and testing groups,<br />intraperitoneal injection of carbon tetrachloride along with daily gavage of B. vulgaris leaf extract 40,<br />80, and 120 mg/kg of weight, respectively. After 6 weeks, the following were checked: enzyme level<br />of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP),<br />as well as serum level of total protein (TP), albumin (ALB), and histopathological status of the liver.<br />RESULTS: The level of AST, ALP, and ALT was increased to 109 (IU/L), 95(IU/L), and 71(IU/L),<br />respectively, in hepatotoxic control group than healthy control group, and there was a decrease of<br />0.86 (g/dl) and 0.04 (g/dl) in TP and ALB levels, respectively. The B. vulgaris extract in every three<br />doses caused a significant decrease in hepatic enzymes level.   However, the TP had a significant<br />increase in 80 and 120 mg/kg of body weight. Regarding ALB, there was no significant difference<br />among these groups. The histopathological results were not conformed to biochemical findings.<br />CONCLUSION: Using the appropriate dose of B. vulgaris leaf extract can help the improvement of<br />laboratory symptoms of fatty liver.<br /><br /> https://jehp.mui.ac.ir/article_27950_950c011d814448023c466411a35b6c62.pdf